Protective Effects of Betulinic Acid on Pentylenetetrazol-Induced Seizures in Rats

Document Type : Original article


Introduction: Seizure is one of the most important diseases of the Central Nervous System (CNS) that affects about 1% to 2% of humans. Seizure is
caused due to a symmetrical electrical discharge in a group of neurons in the CNS. Betulinic Acid (BA) is known as a potent antioxidant with different
effects that have been mentioned in many research studies. In this study, the impact of BA was evaluated on pentylenetetrazol-induced seizure.
Materials and Methods: In this study, 48 male Wister rats were used weighing between 215 and 250 g. The animals were randomized into six
groups, each consisting of eight rats. Treatment groups were treated with different doses of BA (25, 50, and 100 mg/kg); positive and negative control
groups received phenobarbital (80 mg/kg) and normal saline (10 mg/kg), respectively, 30 minutes before pentylenetetrazol injection (50 mg/kg,
intraperitoneally). Then, parameters such as convulsion time, latency to convulsion, pain, passive avoidance memory, and antioxidant status were
Results: The results revealed that BA possesses dose-dependent influence and the dose of 100 mg/kg has the maximum effect for increasing the
latency to convulsion and reducing the convulsion time. Moreover, BA significantly reduced oxidative damage in the rats’ brain tissue compared with
the PTZ-kindled group.
Conclusion: Based on the results obtained in this study, BA can effectively control pentylenetetrazol-induced seizures.